Random mutagenesis

To create proteins with new or improved properties, directed protein evolution is one of the most powerful strategies. Mutagenex provides mutant libraries with a high diversity and accuracy generated by an error-prone PCR and  site-directed random mutagenesis (site saturation) at either single or multiple sites.

Site-directed random mutagenesis is also called targeted random mutagenesis or site-saturation mutagenesis. Since most of conventional directed evolution strategies such as error-prone PCR and DNA shuffling generate large numbers of non-functional mutants rather than functional genes, these approaches inevitably require extensive high-throughput screening system, which request a hugh cost and long time.

By targeting specific residues, most of codons remain as wild-type that can make mutants maintain natural structure of protein backbone despite of hyper diversity of functional region. Therefore, the mutant library can contains high portion of functional mutants that are more likely to yield positive results.

How can we assist you ?

Mutagenex can introduce random or saturated mutations into target sites of gene according to customer's request with high efficiency of mutagenesis, high accuracy without undesired mutations, and a big library size enough for customer's research.

Applications

Site-directed random mutagenesis has been successful for many directed evolution experiments. The applications of this method include,

EnzymeAntibody
Alteration of enontioselectivity
Alteration substrate specificity
New catalytic activity
Stability at extreme temperature and pH
Enhanced binding activity and stability
Decreased immunogenesity
Increased serum half-life

HormoneOther applications
Improved association rate or affinity
Reduced affinity to increase recycling rate
Increased serum half-life
Improvement of cytokines
Alteration of GFP fluorescence property
DNA or RNA sequence optimization

The price will be determined depending on target gene size, number of targeting sites, number of target codons (bases or residues) for randomization, total diversity of random mutation, size of primary library and final quality of library.

We provide a mutant library with a high diversity by error-prone PCR as well as site-specific random mutagenesis (site saturation).

To start your site-directed random mutagenesis project, please contact us.